Application

Western blotting: 1:500-1:3,000 using ECL. Reacts with the ~190 kD Huntingtin protein in unstimulated human PC-3 whole cell lysate. Additional bands at ~130 may also be detected.

ELISA: 1:10,000-1:40,000

Optimal working dilutions must be determined by the end user.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Detect Huntingtin using this Anti-Huntingtin Antibody, phosphoSer 421 validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: phosphoSer 421

Synthetic peptide from human Huntingtin protein. It is expected that the antibody may cross react with rat and mouse due to conservation of the immunogen sequence (8/9).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, 0.15M Sodium Chloride with 0.01% sodium azide.

Specificity

Recognizes Huntingtin protein phosphorylated at serine 421.Minimal reactivity is expected with the non-phosphorylated form of the protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.